MANUAL ECM2001 PDF

JavaScript seems to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website. The Legacy ECM multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories. Not for use in diagnostic, pre-clinical, or clinical procedures.

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JavaScript seems to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website. The Legacy ECM multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.

Not for use in diagnostic, pre-clinical, or clinical procedures. The Legacy ECM is capable of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion. Electrofusion is carried out by applying both the AC and DC pulse waveforms. Microsecond switch-over time from AC to DC allows for highly efficient fusion. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse for the rounding off process; resulting in a higher number of hybrids.

The 1 MHz AC pulse aligns cells together in real time, saving time seconds vs. Blastomeres and oocytes can also be aligned to the correct position for better fusion accuracy during nuclear transfer and embryo manipulation.

Utilizing the Legacy ECM for standard mammalian cell transfections is simple; the AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 9 pulses per experiment, make this an extremely versatile system for any lab.

Electroporate adherent cells directly into the dish used for cell growth. The Legacy ECM coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in.

The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for mm petri dishes. For use in hybridoma production applications, the Legacy ECM generator, 3. This system does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Electrofusion is achieved by the generation of a proprietary 1 MHz AC waveform for benign dielectrophoretic cell alignment, microsecond switchover from AC to DC creates efficient fusion results.

After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse resulting in a higher number of hybrids. Cell Fusion Applications: Electrofusion vs. Natalie J. Lampley, Robin G. Yoder, Michael T. Rock, C. Buddy Creech, Kathryn M. Albrecht, and James E. Crowe Jr. H7N9 influenza virus neutralizing antibodies that possess few somatic mutations. The Journal of Clinical Investigation, April A novel micropit device integrates automated cell positioning by dielectrophoresis and nuclear transfer by electrofusion.

Biomed Microdevices. Green, A. Cattle cloned from increasingly differentiated muscle cells. Biol Reprod. Young Chung, Sandy Becker. Methods in Enzymology. Kenichiro, I. Induction of cytotoxic T lymphocytes against human cancer cell lines using dendritic cell-tumor cell hybrids generated by a newly developed electrofusion technique.

Nakamura, Y. Molecular And Cellular Biology , Soares, M. BMC Developmental Biology, Bedford, S. Yul, Z. Grabarek, J. Liu, H. Human Reproduction, This website requires cookies to provide all of its features. For more information on what data is contained in the cookies, please see our Privacy Policy page. To accept cookies from this site, please click the Allow button below.

More Views. Multi-purpose electro cell manipulation generator capable of producing a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.

Request a Demo. Request a Quote. Details The Legacy ECM multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion. Cell fusion Nuclear transfer Embryo manipulation Hybridoma production Mammalian cell transfection Plant protoplast fusion Stem cell production.

Quick Links. View Price. Adherent Cell Electrode Kit, 5 mm gap, includes cable. Glass Microslides with rectangular electrodes, 10 mm gap, 2.

Glass Microslides with rectangular electrodes, 3. Glass Microslides with round wire electrodes, 0. Glass Microslides with round wire electrodes, 1. Oocyte Electrode, Platinum Plated, 10 mm, 1 mm gap Electrode only. Oocyte Electrode Kit, Platinum Plated, 10 mm, 1 mm gap, with cables. Platinum Tweezertrode Kit, 1 mm Flat includes cable. Platinum Tweezertrode, 1 mm Diameter includes cables. Platinum Tweezertrode, 3 mm Diameter includes cable.

Platinum Tweezertrode, 5 mm Diameter includes cables. Platinum Tweezertrode, 7 mm Diameter includes cables. Stainless Tweezertrode Kit, 10 mm includes cable.

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