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This study intended to purify and characterise exo-inulinase of diesel-degrading Paenibacillus sp. The whole genome sequencing of Paenibacillus sp. This isolate was capable of producing a maximum of An exo-inulinase of Catalytic constant, k cat was calculated to be The results of amino acid alignment and the complete degradation of inulin into fructose by the purified enzyme confirmed that inulinase from Paenibacillus sp.

D9 is an exo-form. The phylogenetic tree based on the protein sequences indicates that bacterial exo-inulinases possess a common ancestry.

Keywords: Characterization; Enzyme purification; Exo-inulinase; Paenibacillus sp. This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features! Clipboard, Search History, and several other advanced features are temporarily unavailable. Search: Search. Advanced Clipboard. Create file Cancel. Email citation To:. Format: Summary Summary text Abstract Abstract text. Send email Cancel. Add to Collections Create a new collection Add to an existing collection.

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Full-text links Cite Favorites. Abstract This study intended to purify and characterise exo-inulinase of diesel-degrading Paenibacillus sp. Similar articles Gene cloning, expression, and characterization of an exo-inulinase from Paenibacillus polymyxa ZJ Gao J, et al.

Appl Biochem Biotechnol. Epub May 8. PMID: Cold-active and NaCl-tolerant exo-inulinase from a cold-adapted Arthrobacter sp. MN8 and its potential for use in the production of fructose at low temperatures. Zhou J, et al. J Biosci Bioeng. Epub Sep Expression of exo-inulinase gene from Aspergillus niger 12 in E. Yedahalli SS, et al. Biotechnol Prog. Epub Feb Molecular characterization and expression of microbial inulinase genes.

Liu GL, et al. Crit Rev Microbiol. Epub Jun PMID: Review. Biotechnological potential of microbial inulinases: Recent perspective. Rawat HK, et al. Crit Rev Food Sci Nutr. Show more similar articles See all similar articles.

References Nutr Rev. Publication types Research Support, Non-U. Gov't Actions. Hydrogen-Ion Concentration Actions. Phylogeny Actions. Substances Bacterial Proteins Actions.

Glycoside Hydrolases Actions. Full-text links [x] Springer. Copy Download.


Purification and Characterization of Exo-Inulinase From Paenibacillus Sp. d9 Strain

Optimum pH for inulinase activity I was between 4. The optimum temperature was 60 O. C for both activities and no loss in activity was observed when it was maintained at this temperature for 30 min. The K m value was 1.


We'd like to understand how you use our websites in order to improve them. Register your interest. The extracellular inulinase of the marine yeast Pichia guilliermondii strain 1 was purified to homogeneity resulting in a 7. The molecular mass of the purified enzyme was estimated to be The optimal pH and temperature for the purified enzyme were 6. The K m and V max values of the purified inulinase for inulin were

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