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Influence of temperature on the reproduction of the earthworm Eisenia foetida Acute toxicity of chemical pesticides and plant-derived essential oil on the behavior and development of earthworms, Eudrilus eugeniae Kinberg and Eisenia fetida Savigny. Comparative toxicity of two chemical pesticides temephos and monocrotophos versus a plant-derived betel leaf oil Piper betle L.

In contrast, P. The lethal concentration LC 50 value was observed at 3. Correspondingly, the avoidance or attraction assay also displayed that earthworms were more sensitive to the soil containing chemical pesticides. Whereas, the avoidance percentage was decreased in the P. Similarly, sublethal concentration of chemical pesticides 5 and 6. However, P. Hence, our study implied the risk assessment associated with the chemical pesticides and also recommends plant-derived harmless P.

Comparative toxicity of tetra ethyl lead and lead oxide to earthworms, Eisenia fetida Savigny. Leaded gasoline contains tetra ethyl lead TEL as an antiknocking agent, which produces major amounts of lead oxide in automobile exhaust along with traces of TEL. To minimize the lead contamination, methyl tertiary butyl ether MTBE is used as a substitute for producing unleaded gasoline. It has become increasingly apparent that young children are highly susceptible to the harmful effects of lead.

Hence, a study was carried out to monitor lead toxicity in soil, using adult earthworms Eisenia fetida, Savigny. Leaded gasoline TEL and lead oxide are and fold more toxic than unleaded gasoline MTBE in 7 days of exposure and and fold more toxic in 14 days, respectively. Results indicate that the presence of TEL in leaded gasoline and lead oxide has a significant effect on behavior, morphology, and histopathology of earthworms.

Absorption of TEL into the tissues is comparatively less than that of lead oxide but toxic effects were severe. Rupture of the cuticle, extrusion of coelomic fluid and inflexible metameric segmentation were observed, causing desensitization of the posterior region leading to fragmentation in earthworms. Acute and chronic toxicity testing of TPH-contaminated soils with the earthworm, Eisenia foetida.

Responses of Eisenia foetida to petroleum-contaminated soils are being assessed using a day test described previously. The authors prepared dilutions of two soils, referred to as A and B, using their reference-soil counterparts, collected from near the contaminated sites. The total petroleum hydrocarbon TPH content of each soil was measured by latroscan before the dilutions were prepared.

Thus, neither reference soil was pristine. Dilutions of the A soil tested with E. Mean growth of Eisenia in dilutions of the A soil ranged from 48 to 74 mg dry-weight growth per pair of worms; these values were lower than those in any dilution of the B soil series.

Lipid levels of worms in higher concentrations of the A and B soils were similar to one another and to published values, suggesting little inhibition of feeding in either dilution series. Earthworm reproduction was zero in the A series, but moderately high in the B series. Thus, the A soil apparently contained materials other than TPH that inhibited earthworm growth and reproduction. This study shows that 1 TPH at concentrations as high as 1, ppm may not always be inhibitor to earthworm growth or reproduction and 2 that earthworm survival, as a test endpoint, is much less sensitive than either growth or reproduction.

Biodegradation of paper waste using Eisenia foetida by vermicomposting Technology. The paper wastes are being a big concern over past decades. The paper waste in SASTRA is collected around 50kg and organic wastes like vegetable wastes and cow dung wastes are also collected. The area is divided into three segments and in each segment appropriate amount of paper waste and organic waste were added along with 25 numbers of earthworms.

The setup is watered daily and monitored periodically and it is kindled for proper aeration. The soil samples were collected on 20 days, 45 days and 60 days from the day the earthworms were added. After 60 days of the experiment, the paper wastes, compost and earthworms are separated. The quantity of the wastes was compared to the initial amount and the composts are collected.

The elemental analysis of the soil used as Vermi-bed is analyzed for improvement of soil nutrients. The vermiwashed water of the setup is analyzed for total protein. The number of earthworm is also compared to initial quantity. Out of all, the loss percentage of the organic waste and paper waste shows the degradation of the paper wastes. Toxicity of azodrin on the morphology and acetylcholinesterase activity of the earthworm Eisenia foetida.

The acute toxicity of azodrin monocrotophos, an organophosphorus insecticide was determined on a soil organism, Eisenia foetida. The median lethal concentrations LC 50 were derived from a h paper contact test and from artificial soil tests. The LC 50 of azodrin in the paper contact test was 0.

The neurotoxic potentiality of azodrin was assessed by using a marker enzyme, acetylcholinesterase AChE; EC 3. The progressive signs of morphological destruction are correlated with percentage inhibition of AChE in the in vivo experiments. The kinetics of AChE activity in the presence and absence of azodrin indicated that the toxicant is competitive in nature.

This study demonstrated that azodrin causes concentration-dependent changes in the morphology and AChE activity of the earthworm E. Directory of Open Access Journals Sweden. The maximum V. In the second stage, the survival of Eisenia foetida in the anaerobic sewage sludge was investigated.

In the unmixed raw anaerobic sludge, all the earthworms died during the first 9 weeks of the study period due to acute toxicity. From week 10, however, their survival rate improved so that by week 12 when toxicity reduced to This is while in the mixture of anaerobic sludge with paper D.

Toxic responses of Sox2 gene in the regeneration of the earthworm Eisenia foetida exposed to Retnoic acid. Exogenous retinoic acid delays and disturbs the regeneration of Eisenia foetida.

The stem cell pluripotency factor, Sox2, can play a crucial role in cell reprogramming and dedifferentiation. In this study, we compared the regeneration of Eisenia foetida in different segments after amputation and the effects of retinoic acid on the regeneration of different segments.

The results showed that the regeneration speed of the head and tail was slightly faster than the middle part, and retinoic acid disrupted and delayed the regeneration of the earthworm.

The qRT-PCR and Western blot analysis showed that the expression of the Sox2 gene and Sox2 protein was highest on the seventh day in different segments pregeneration of earthworms and the formation of blastema are related to the expression of the Sox2 gene and protein.

Retinoic acid delays and interferes with the regeneration of the earthworm by affecting the expression levels of the Sox2 gene and protein. All rights reserved. Full Text Available Copper toxicity in soil was evaluated using biomarkers of oxidative stress catalase enzyme activity, superoxide dismutase and lipid peroxidation in the earthworm Eisenia foetida. Agricultural topsoils from mining areas of the Aconcagua river basin were collected.

Parameters of antioxidant activity were more sensitive than the weight change and thus can be used as appropriate biomarkers in Eisenia foetida.

Use of the reference organism Eisenia foetida to investigate bioaccumulation and biological effects following contamination of soil by uranium. Full text of publication follows: The use of reference organisms for radiological assessments on non -human species is an integral part of the current systemic approach for the management of radiation effects in the environment.

The reference organisms approach allows the evaluation of radiological impact on the environment taking into account relationships among ambient radionuclide activity concentrations, dose and expected adverse biological effects.

Four broad categories of biological damages are included: mortality, morbidity, DNA damage and reproductive failure. Earthworms are one of the most important biotic components in the soil, they are commonly used in studies of toxicity and they are included in the list of the reference organisms suggested by International ICRP and national organisations. However, up to now, no adequate results have been obtained for earthworms allowing the identification of the dose-response relationship, essentially for the contamination scenarios where radionuclide can provide both radiation and chemical impact.

Uranium U is a naturally occurring heavy metal. Recently there has been public concern on the presence in the environment of depleted uranium DU , a by-product of the process used to enrich natural uranium ore for use in nuclear reactors and in nuclear weapons.

The presence of uranium in soil could lead to both toxic and radiation impact and it is difficult to distinguish the different impacts and their contribution to possible biological effects. European Union, OECD and FAO have selected the earthworms Eisenia for testing soil toxicity because it is an organism that can be easily cultured in the laboratory, an extensive database is available, and it feeds at the soil surface level.

The prime objective of the present study was to evaluate the possible use of Eisenia foetida as a bio-marker of U environmental impact. Four groups of six sexually mature Eisenia foetida were maintained in the dark at 21 deg.

C in Petri. Mortality of the earthworms, Eisenia foetida , after gamma-irradiation at different stages of their life history. Effects of ionizing radiation on the earthworm, Eisenia foetida , were investigated by means of mortality, growth inhibition and hatchability of the eggs.

Young earthworms were less resistant than adult ones. Mature earthworms were irradiated and the hatchability of the cocoons laid by them after irradiation was examined. Hatchability of the cocoons laid within 5 days after irradiation with 2 kR was scarcely affected. Then the hatchability decreased with the interval between irradiation and laying, and reached lowest level 20 days after irradiation. From 20 days after irradiation, it started to recover and reached the control level 30 days after irradiation.

Could humic acid relieve the biochemical toxicities and DNA damage caused by nickel and deltamethrin in earthworms Eisenia foetida?

The aim of the study was to determine whether humic acid HA prevented gene and biochemical toxic effects in earthworms Eisenia foetida exposed to nickel and deltamethrin at and 1 mg kg -1 , respectively in soil.

Cellular- and molecular-level toxic effects of nickel and deltamethrin in earthworms were evaluated by measuring damage to lipid membranes and DNA and the production of protein carbonyls over 42 days of exposure. The presence of HA alleviated in decreasing order of effectiveness damage to DNA, proteins, and lipid membranes caused by nickel and deltamethrin.

A low HA dose 0. However, the malondialdehyde concentrations in earthworms were higher at the highest HA dose than at the lower HA doses. The amounts of protein carbonyls produced at different HA doses were not significantly different. The toxic effects to earthworms caused by increased oxidizable nickel concentrations could be relieved by adding HA. Lytic activities in coelomic fluid of Eisenia foetida and Lumbricus terrestris. Coelomic fluids of the two earthworm species E.

Both activities are carried by different molecules since hemolysis but not proteolysis was inhibited by simple sugars. In contrary, proteolysis was blocked by PMSF which did not influence hemolysis.


Chemical Composition of Earthworm (Eisenia foetida) Co-Dried with Vegetable Meals as an Animal Feed

To evaluate the chemical composition of the earthworm Eisenia foetida co-dried EW with vegetable meals VM as animal feed ingredient, the blends were mixed with wheat bran WB , rice powder RP , corn meal CM and soy cake meal SCM in proportions of ; and However, the use of vegetable meals proportionally increased the CF 7. It is concluded that the most appropriate proportion VM:EW will depend on the animal species, productive stage and market requirement. This work is licensed under a Creative Commons Attribution 4. Sistema OJS3 - Metabiblioteca. Quick jump to page content.


Eisenia andrei

Assessment of copper toxicity in soils using biomarkers of oxidative stress in eisenia foetida. Copper toxicity in soil was evaluated using biomarkers of oxidative stress catalase enzyme activity, superoxide dismutase and lipid peroxidation in the earthworm Eisenia foetida. Agricultural topsoils from mining areas of the Aconcagua river basin were collected. Parameters of antioxidant activity were more sensitive than the weight change and thus can be used as appropriate biomarkers in Eisenia foetida. Keywords: catalase; superoxide dismutase. El cobre es un importante contaminante ambiental asociado, en el caso de Chile, principalmente a las actividades mineras.

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